Ribonucleic Acid Regulation in Permeabilized Cells of Escherichia coli Capable of Ribonucleic Acid and Protein Synthesis
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چکیده
منابع مشابه
Regulation of ribonucleic acid synthesis in Escherichia coli during diauxie lag: accumulation of heterogeneous ribonucleic acid.
The synthesis of ribonucleic acid (RNA) and of protein in Escherichia coli during glucose-lactose diauxie lag have been examined. The rate of RNA synthesis is about 7%, of the corresponding rate during exponential growth and the rate of protein synthesis 10 to 15%. Inhibition of RNA synthesis occurs to the same extent in both rel and rel(+) strains. The RNA which accumulates during 20 min in di...
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In trimethoprim-inhibited RCStr strains of Escherichia coli, the expression of the RC control of stable RNA synthesis arose primarily from a decrease in the intracellular concentrations of glycine and methionine, and not from inhibition of the initiation ofnew protein chains. In non-supplemented cultures, experiments with rifampicin showed that the immediate response to the addition of trimetho...
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1. A cell membrane-DNA-RNA polymerase complex which contains virtually all of the RNA-synthetic capacity of the cell has been isolated from uninfected and R23-infected Escherichia coli. 2. Infection of E. coli by RNA bacteriophage R23 inhibits the RNA synthetic capacity of the membrane complex. In infected cells some of the RNA synthetic capacity is released from the membrane fraction. 3. The m...
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At present it is thought that adenosine triphosphate-mediated activation of the ol-carboxyl group of amino acids is a necessary step in the synthesis of at least some bacterial proteins. Evidence for the obligatory role of this activation has been available mainly from studies in vitro of protein synthesis (e.g. Simpson (2)), but a mutant of Escherichia coli has recently been isolated (1) which...
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Ribosomes and immature ribonucleoprotein particles were isolated from extracts of log-phase cells grown under various conditions. Quantitative measurements were made to determine the relative amounts of immature particles present in the extracts. The results indicate that the steady-state level of ribosomal precursors accounted for essentially a constant fraction of the total ribonucleic acid (...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1974
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.118.3.1186-1189.1974